منابع مشابه
Immune monitoring technology primer: flow and mass cytometry
Description of the technology Flow cytometry traditionally uses fluorochrome-labeled probes, such as antibodies, to identify cells expressing the targets of those probes. A sample stream carries single cells in suspension past a laser, exciting the fluorochromes, with quantitation of the emitted fluorescent signals from each cell via optical filters and photomultiplier tubes [1]. In mass cytome...
متن کاملflow cytometry applications in the study of immunological lung disorders.
the use of flow cytometry in the clinical laboratory has grown substantially in the past decade. flow cytometric analysis provides a rapid qualitative and quantitative description of multiple characteristics of individual cells. for example, it is possible to detect the cell size and granularity, aspects of dna and rna content and the presence of cell surface and nuclear markers which are used ...
متن کاملMonocytes in metabolic disorders--opportunities for flow cytometry contributions.
Chronic inflammation has arised as a major underlying cause of atherosclerosis, obesity and diabetes. It is mediated by cells of innate immune system like macrophages but also by their antecedents, circulating monocytes. Roles of monocyte subsets and different markers of monocyte activation in the context of metabolic disorders have been reviewed. Applying cell based approach through flow cytom...
متن کاملImmune Response to Mycobacterial Infection: Lessons from Flow Cytometry
Detecting and treating active and latent tuberculosis are pivotal elements for effective infection control; yet, due to their significant inherent limitations, the diagnostic means for these two stages of tuberculosis (TB) to date remain suboptimal. This paper reviews the current diagnostic tools for mycobacterial infection and focuses on the application of flow cytometry as a promising method ...
متن کاملManaging Multi-center Flow Cytometry Data for Immune Monitoring
With the recent results of promising cancer vaccines and immunotherapy1-5, immune monitoring has become increasingly relevant for measuring treatment-induced effects on T cells, and an essential tool for shedding light on the mechanisms responsible for a successful treatment. Flow cytometry is the canonical multi-parameter assay for the fine characterization of single cells in solution, and is ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2012
ISSN: 1552-4922
DOI: 10.1002/cyto.a.22207